Pavel KraikivskiResearch Scientist Department of Biological Sciences Virginia Tech Blacksburg, VA 24061-0406 Email: email@example.com Tel: 540-231-6398 Fax: 540-231-9307 Research Pages:
Current Research Interests
Top-down and bottom-up approaches in modeling cell cycle regulation. Cell cycle control system is often modeled as a complex network of such interacting elements as cyclins, kinases, phosphatses and transcription factors. Almost a decade ago, the basic principles of the cell cycle and a kind of temporal control mechanism for such events as DNA synthesis, mitosis and cytokinesis were formulated. However, scientists around the globe have never stopped searching for key proteins, transcription factors, genetic and physical interactions regulating cell cycle. Now, we have plenty of data in various databases but still the scarcity of their explanation, interpretation and meaning. In my current project, we use two approaches to this problem: top-down approach and bottom-up approach. The top-down approach mines high-throughput ‘omic-databases’ for interactions and correlations that are relevant to a cell cycle. This connections are usually displayed as graphs that not only encode considerable information but also suggest new way of thinking about cell regulation. The bottom-up approach builds a detailed kinetic model of the biochemical mechanism of a specific control system, and solves dynamic equations to predict the time-course of every component of the system.
I am also interested in the self-assembly and dynamics of cytoskeletal filaments like actin filaments and microtubules. Many of these systems arise from organization of biopolymers into a defined pattern or network which has unique mechanical properties rigid enough to maintain shape and transmit forces, yet flexible enough to allow internal reorganization. Dynamics of such intracellular structures is driven by thermal fluctuations as well as such active processes as nucleation, polymerization, depolymerization, crosslinking of filaments and their active transport by motor proteins.
Another research area is intracellular transport, a process fundamental for cellular function, survival and morphogenesis. For this project, the model system that is being used is melanophores, pigment cells of lower vertebrates. The only function of these large cells is synchronous transport of thousands of membrane-bounded organelles, pigment granules. This system is ideal for both modeling and experimental study of intracellular transport control system. My research is focused on the role of cytoskeleton in intracellular transport and regulation of activity of motor molecules by signal transduction mechanisms.
|Ph.D.||2005||Max-Planck Institute for Colloids and Interfaces, Potsdam University, Germany, Biophysics.|
|M.S.||2002||Ioffe Physical-Technical Institute, Saint Petersburg State Polytechnical University, Russia, Physics.|
|B.S.||2000||Saint Petersburg State Polytechnical University, Russia, Physics.|
|2011 - Present||Research Faculty, Department of Biological Sciences, Virginia Tech. Cell cycle control, (Prof. John Tyson lab).|
|2006 - 2011||Postdoctoral Fellow, Center for Cell Analysis and Modeling, Department of Cell Biology, University of Connecticut Health Center. Biological pathway quantification, intracellular transport, kinetics of actin dendritic nucleation, cell motility, Cytoskeletal Filaments, actin bundling, diffusion in crowded cytoplasm, and contribution to VCell development. (Prof. L.M. Loew laboratory, Prof. B.M. Slepchenko, and Prof. V. Rodionov).|
|2002 - 2006||Ph.D. Candidate. Max-Planck Institute for Colloids and Interfaces, Germany. Semiflexible polymers adsorbed on structured substrates. Collective ilament dynamics in motility assays for motor proteins. Short- and long- working distance optical trappings. (Prof. R. Lipowsky, Prof. Jan Kierfeld and Dr. R. Dimova).|
|1999 - 2002||Research Technician. Institute for Analytical Instrumentation Russian Academy of Science, Russia. Electrospray ionization mass spectrometry, optical emission spectroscopy. (Prof. L.N. Gall laboratory).|
|1997 - 2002||Research Technician. Ioffe Physical-Technical Institute of the Russian Academy of Sciences, and St.-Petersburg State Technical University, Russia. Glassy chalcogenide semiconductors, electron paramagnetic resonance spectroscopy. (Prof. K. D. Tsendin and Prof. K.F. Shtel’makh laboratories).|
|For a detailed description of Pavel' research interest and professional experience, please visit his research website.|
Pavel is from St. Petersburg, the most beautiful city in Russia. When not working, Pavel likes to spend time playing with his son. He is also keen on traveling, hiking and mountain skiing.