Modeling the Budding Yeast Cell Cycle

Budding Yeast Model

Simulation:

Numerical solution of the mathematical model for the wild type budding yeast is shown.

Simulations compared well with experimental observations.

For a culture growing exponentially at a MDT=90 min,

(1) Duration of cell cycle phases:


  Cycle time, min G1 length, min S/G2/M, min
Daughter Cell 97.5 (101.2)* 42 (36) 57 (64)
Mother Cell 81 (80) 22 (28) 59 (52)

* Data for a wild type yeast diploid strain A364A D5 are obtained from Brewer et al., 1984, simulation results are shown in parenthesis.

(2) The relative amounts of cyclins and CKIs:

([Cln1]+[Cln2]) : (Clb5]+[Clb6]) : ([Clb1]+[Clb2]) : [Sic1] : [Cdc6]
=15 : 3.8 : 7.5 : 1 : 3 (in experiments)
=15 : 3.3 : 4.7 : 2.8 : 3.7 (in model).

Measurements are made by Cross et al., 2002, and Archambault et al., 2003.