Modeling the Budding Yeast Cell Cycle

GAL-CLB5 sic1∆

Simulation:

Change of parameters: ksb5'=0.012, ksc1'=ksc1"=0, MDT=150.
Arrest: It dies after the first cycle, Ori not relicensed.

Experiments:

Jacobson, M.D., Gray, S., Yuste-Rojas, M. and Cross, F.R. (2000). Testing cyclin specificity in the exit from mitosis. Mol. Cell. Biol. 20:4483-4493.
[Abstract] [Article]
Experimental results: Lethal, data not shown.
Comments: It can exit the first mitosis but [ORI] (grey line) is not relicensed, due to high Clb5 kinase activity in G1.