Modeling the Budding Yeast Cell Cycle

cdc14ts then GAL-SIC1

Simulation:

Change of parameters: ks14=0 for 180 min till telophase arrest, then 3 copies of GAL-SIC1(ksc1'=0.39, MDT=150).

Experiments:

Fitzpatrick, P.J., Toyn, J.H., Millar, J.B. and Johnston, L.H. (1998). DNA replication is completed in Saccharomyces cerevisiae cells that lack functional Cdc14, a dual-specificity protein phosphatase. Mol. Gen. Genet. 258:437-441.
[Abstract] [Article]
Experimental results: Able to exit from mitosis.
Comments: Exit of mitosis 12 min after turning on GAL-SIC1.