Modeling the Budding Yeast Cell Cycle

swi5∆ cdh1∆

Simulation:

Change of parameters: ksswi'= ksswi"=0, kscdh=0, init CDH1=CDH1T=0.
Arrest: Inviable, telophase arrest.

Experiments:

Archambault, V., Li, C.X., Tackett, A.J., Wasch, R., Chait, B.T., Rout, M.P. and Cross, F.R. (2003). Genetic and biochemical evaluation of the importance of Cdc6 in regulating mitotic exit. Mol. Biol. Cell 14:4592-4604.
[Abstract] [Article]
Experimental results: Similar to the triple-antagonist. They were able to undergo DNA synthesis and nuclear division, but not cell division and arrested as binucleate cells with 4C DNA content.
Comments: Problem for the model. The double mutant swi5∆ cdh1∆ has similar phenotype to the triple-antagonist, because with Swi5 deletion, Sic1 and Cdc6 synthesis are drastically reduced. And our model is at odds with this mutant as well.