- Pds1 / Esp1 Mutants
- pds1∆
- pds1∆ in nocodazole
- bub2∆ pds1∆ in nocodazole
- mad2∆ pds1∆ in nocodazole
- cdc20∆ pds1∆
- cdc20∆ pds1∆ clb5∆
- CLB5-db∆ pds1∆
- CLB5-db∆ pds1∆ cdc20∆
- CLB1 clb2∆ pds1∆
- esp1-ts
- GAL-PDS1-db∆ esp1-ts
- GAL-PDS1-db∆
- PDS1-db∆
- GAL-ESP1 cdc20-ts
pds1∆
debug: ,
test user =
test db =
Simulation:
Change of parameters: ks1pds"=ks2pds"=0.
Length of G1 phase: 40.2 min.
Mass at division: 2.25
Experiments:
Yamamoto, A., Guacci, V. and Koshland, D. (1996). Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s). J. Cell Biol. 133:99-110.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Fig. 3 and Table 1, viable.
webmaster
What seems to prevent premature chromosome segregation in pds1∆ is that Cdc5 kinase is required for efficient cleavage of Scc1 by Esp1 (Alexandru et al., 2001) and for efficient dissociation of securin from chromosome arms (Nasmyth, 2001). Presumably Cdc5 is activated by Clb2 kinase only after a significant delay, long enough for chromosomes to align on the metaphase plate. Hence, PDS1 is not an essential gene.
If this role of Cdc5 were incorporated in the model, the problem with pds1∆ might disappear.