- Cdc20 Mutants
- cdc20-ts
- cdc20∆ clb5∆
- cdc20∆ pds1∆
- cdc20∆ pds1∆ clb5∆
- CLB5-db∆ pds1∆ cdc20∆
- cdc20-ts bub2∆
- cdc20-ts mad2∆
- net1∆ cdc20-ts
- GAL-ESP1 cdc20-ts
- GAL-CDC20
- APC-A cdh1∆ multicopy CDC20
- sic1∆ cdh1∆ GALL-CDC20
- sic1∆ cdc6∆2-49 cdh1∆ GALL-CDC20
net1∆ cdc20-ts
debug: ,
test user =
test db =
Simulation:
Change of parameters: kasrent=1, kasrentp=0.005, ks20'=ks20"=0.
Arrest: Mitotic exit at t=196 min with sister chromatids still attached.
Experiments:
Visintin, R., Hwang, E.S., Amon, A. (1999). Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. Nature 398:818-823.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Table 1, inviable.
Comments: Sister chromatids do not separate (Pds1 is high at exit of mitosis).
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