Data comparison among simulated profiles and experimental data
Figure 6. Comparison of simulated protein time profiles and DNA accumulation (curves) with experimental data (circles).
(A) The simulated total DNA (green), Elongation (blue) and Initiation (red) variables. The experimental data for total DNA are taken from Figure 4 in (Dingwall and Shapiro, 1989).
(B) Curves are simulated probabilities of hemimethylated states of Cori and three genes. The appearance of hemimethylated gene sites in our simulation reflects the nearly linear growth of overall DNA-hemimethylation observed experimentally (data points (black) from Figure 3 in (Stephens et al., 1996)). The peak of hemimethylation (~75 min) in our simulation agrees well with experimental observation.
(C) Time courses of GcrA (blue) and CtrA (red) are compared against experimental data from Figure 3 in (Holtzendorff et al., 2004) and Figure 1 in (Grunenfelder et al., 2001), respectively. Fluctuations of DivK~P (green) are discussed in the text.
(D) The generalized Fts protein time course (blue) is compared to the measured profile of FtsQ from Figure 2 in (Martin et al., 2004). Our simulation of Z-ring constriction (red curve) is consistent with the ~20 min closure time reported in (Judd et al., 2003), Figure 2. CcrM (black) accumulates to high level only in the late predivisional cell, in agreement with the data (black) in (Grunenfelder et al., 2001), Figure 2. DnaA data (green) from Figure 5 in (Zweiger and Shapiro, 1994) do not show significant variations during the stalked cell cycle, consistent with our simulation (green curve).
(E) The relative activities of ctrA promoters, ctrA-P1 (red) and ctrA-P2 (blue), as reported in Figure 4A in (Reisenauer and Shapiro, 2002), compares well with our simulation (red and blue curves).
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