Predictions (iii):
The mutant GAL-CLB5 is alive ( Schwob & Nasmyth, 1993), which depends on the inhibition of Sic1 on Clb5 activity for viability. Since Cdc6 is not an efficient inhibitor of Clb5, deletion will not affect the viability of GAL-CLB5.
Genotype | Phenotype |
GAL-CLB5 sic1∆ GAL-CLB5 cdc6∆2-49 |
Inviable, inefficient origin licensing Viable, size similar to WT (prediction). |