Modeling the Budding Yeast Cell Cycle

Predictions of the model

#
 Genotype Phenotype
i.

triple-cln

triple-cln sic1∆

triple-cln cdc6∆2-49

Inviable, G1 arrest (Wittenberg et al., 1990).

Viable, large (Tyers, 1996).

Inviable, G1 arrest (prediction).
ii.

cln3∆ bck2∆

cln3∆ bck2∆ sic1∆

cln3∆ bck2∆ cdc6∆2-49

Inviable, G1 arrest (Epstein & Cross, 1994)

Inviable (Wijnen & Futcher, 1999).

Inviable (prediction)
iii.

GAL-CLB5 sic1∆

 

GAL-CLB5 cdc6∆2-49

Inviable, inefficient origin licensing
(Jacobson et al., 2000).

Viable, size similar to WT (prediction).
iv.

CLB5-db∆ sic1∆

 

CLB5-db∆ cdc6∆2-49

Inviable, inefficient origin licensing
(Wasch & Cross, 2002).

Viable, size similar to WT (prediction).
v.

GAL-CLB5-db∆

 

GAL-CLB5-db∆ 2X SIC1

GAL-CLB5-db∆ GAL-SIC1

 

GAL-CLB5-db∆ GAL-CDC6

Inviable, inefficient origin licensing
(Jacobson et al., 2000).

Viable, size similar to WT (prediction).

Viable, size bigger than GAL-CLB5-db∆ 2XSIC1 (prediction).

Still inviable (prediction).
vi.

CLB2-db∆

CLB2-db∆ GAL-SIC1

CLB2-db∆ GAL-CDC6

Arrests in telophase (Wasch & Cross, 2002).

Viable (Cross, 2003).

Viable (prediction).

 
vii.

cdc20∆ clb5∆ pds1∆

triple-deletion + 1.5X NET1
(or 0.5X CDC15, or 0.5X CDC14)

triple deletion + bub2∆

triple deletion + sic1∆
(or cdc6∆2-49, or both)


Viable (Shirayama et al., 1999).

Arrests in telophase (prediction)

 

Arrests in G1 (prediction).

Viable (prediction).
viii.

cdc20∆ pds1∆

cdc20∆ pds1∆ TAB6-1
(or with additional CLB5-db∆)

cdc20∆ pds1∆ 2X CDC15

Arrests in telophase (Shirayama et al., 1999).

Viable (prediction).

 

Viable (prediction).
ix.

APC-A cdh1∆

APC-A sic1∆

APC-A sic1∆ cdc6∆2-49

Inviable in glucose (Cross, 2003).

Viable (Cross, 2003).

Viable (prediction).
x.

sic1∆ cdc6∆2-49 cdh1∆ GALL-CDC20

quadruple mutant + mad2∆

Viable (Archambault et al., 2003).

Inviable, G2 arrest (prediction).
xi.

In nocodazole:

net1-ts

net1-ts 2X CLB2 (or 2X CLB5)

net1-ts 2X CLN2 (or 2X CLN3)

net1-ts sic1∆ (or cdc6∆2-49 or both)

 

Loses viability (Visintin et al., 1999)

Retains viability (prediction).

Loses viability (prediction).

Loses viability (prediction).
xii.

cln1∆ cln2∆ clb5∆ clb6∆

quadruple mutant + GAL-CLB2
(or + cdh1∆)

Inviable, arrests in G1 (Schwob & Nasmyth, 1993).

Able to do DNA synthesis and mitosis, but no bud formation. Becoming dinucleate, similar to cdc24-ts (prediction).