- Cdc6 Mutants
- cdc6∆2-49
- cdh1∆ cdc6∆2-49
- sic1∆ cdc6∆2-49
- sic1∆ cdc6∆2-49 cdh1∆
- sic1∆ cdc6∆2-49 cdh1∆ GALL-CDC20
- swi5∆
- swi5∆ cdh1∆
- swi5∆ cdh1∆ GAL-SIC1
- swi5∆ GAL-CLB2
- CLB2-db∆ GAL-CDC6
- CLB2-db∆ multicopy CDC6
- APC-A cdh1∆ GAL-CDC6
- APC-A cdh1∆ multicopy CDC6
- APC-A sic1∆ cdc6∆2-49
cdh1∆ cdc6∆2-49
debug: ,
test user =
test db =
Simulation:
Change of parameters: ksf6'=ksf6"=ksf6"'=0, kscdh=0, init CDH1T=CDH1=0.
Length of G1 phase: 60.2 min.
Mass at division: 1.96
Experiments:
Calzada, A., Sacristan, M., Sanchez, E. and Bueno, A. (2001). Cdc6 cooperates with Sic1 and Hct1 to inactivate mitotic cyclin-dependent kinases. Nature 412:355-358.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Fig. 3b, the mutants are viable and larger. They show a signifant lag in mitosis. Asynchronously growing cells show a marked increase in the percent of cells with 2C DNA contents as compared with WT.
Comments: Problem for the model. The simulation predicts correctly that the mutant cells are viable, but they are smaller than wild-type and have a shorter G2 phase.
webmaster