Mutant cln1∆ cln2∆ clb5∆ clb6∆ can not make bud, it cannot be rescued by over-expression of Clb2.
cln1∆ cln2∆ clb5∆ clb6∆
quadruple mutant + GAL-CLB2
Inviable, arrests in G1 (Schwob & Nasmyth, 1993).
Able to do DNA synthesis and mitosis, but no bud formation. Becoming dinucleate, similar to cdc24-ts (prediction).